Journal: Scientific Reports

Article Title: Tuning IL-2 signaling by ADP-ribosylation of CD25

doi: 10.1038/srep08959

Figure Lengend Snippet: Splenocytes from DEREG mice were incubated in the absence or presence of the indicated concentrations of NAD + before staining with fluorochrome-conjugated mAbs directed against CD4 and CD25 (mAb PC61 or mAb 7D4). Gating was performed on CD4 + cells. (a) Representative dot plots of cells incubated in the absence or presence of 12 μM NAD + . (b) Percentages of CD4 + GFP + cells staining with anti-CD25 mAbs plotted as a function of the concentration of added NAD + . Results are representative of two independent experiments.

Article Snippet: Alternatively, after erythrocyte lysis with Ack lysis buffer (Bio Whittaker) and depletion of B-cells with Dynabead-conjugated sheep anti-mouse IgG (Invitrogen), Tregs were positively selected by magnetic cell sorting using PE conjugated anti-CD25 (7D4) and magnetic bead-conjugated anti-PE antibodies according to the manufacturer's instructions (Miltenyi Biotec).

Techniques: Incubation, Staining, Concentration Assay

Journal: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Article Title: Functional divergence of antigen-specific T-lymphocyte responses in syngeneic graft-versus-host disease.

doi: 10.1016/j.bbmt.2004.05.003

Figure Lengend Snippet: Figure 2. Flow cytometric selection of CLIP antigen-specific T-cell subsets. Splenic T lymphocytes harvested on day 14 after cessation of cyclosporine treatment were subjected to 3-color immunofluorescence. The cells were stained with the biotinylated soluble MHC class II immunoglobulin construct loaded with the N- or C-terminal CLIP variants (counterstained with CyChrome/streptavidin) and with fluorescein isothiocyanate (FITC)–and phycoerythrin (PE)–conjugated antibodies to CD8 and CD25, respectively. The percentage of cells staining with the construct is shown in the histogram; the dot plot displays CD8 and CD25 expression of the cells reacting with the peptide-loaded construct.

Article Snippet: The shaded area, however, repr etween N- and C-terminal flanking domain dependence. eric molecule loaded with unrelated peptides was [ B & M T neffective in isolating CLIP-specific T cells [23]. low cytometric multicolor analysis was used to isoate the antigen-specific T cells and to confirm expresion of the CD4, CD8, and CD25 cell-surface markrs (phycoerythrin- or fluorescein isothiocyanate– onjugated mouse anti-rat CD8, anti-CD4, and CD25 onoclonal antibodies; Serotec, Harlan Bioproducts or Science, Indianapolis, IN).

Techniques: Selection, Staining, Construct, Expressing

Journal: The Journal of Veterinary Medical Science

Article Title: Anti-Inflammatory Effects of Mannanase-Hydrolyzed Copra Meal in a Porcine Model of Colitis

doi: 10.1292/jvms.13-0424

Figure Lengend Snippet: Effect of MCM on (A) fecal IgA concentrations and (B) plasma CD4+CD25+ cell populations. Data are mean ± SEM; n=3; “*” indicates significant difference from NEG; “ # ” indicates significant difference from POS; P <0.05.

Article Snippet: This cell suspension (50 μl , 10 7 cells/ μl ) was then incubated with mouse anti-pig CD25 (AbD Serotec, Oxford, U.K.), goat anti-mouse IgG1:FITC (AbD Serotec), R-Phycoerythrin-conjugated anti-pig CD4a (BD Biosciences, San Diego, CA, U.S.A.) and anti-mouse/rat FoxP3 Alexa Fluor (eBioscience, San Diego, CA, U.S.A.) according to the manufacturers’ instructions.

Techniques: Clinical Proteomics

Journal: Human Vaccines & Immunotherapeutics

Article Title: Research and development of mAb drugs in China: a look from policy perspectives

doi: 10.1080/21645515.2019.1605809

Figure Lengend Snippet: Domestically approved mAb drugs by 2017.

Article Snippet: , Jiannipai , Recombinant Humanized Anti-CD25 mAbs Injection , Shanghai CP Guojian Pharmaceutical Co., Ltd. , Humanzied , 2011 , Transplant Rejection.

Techniques: Injection, Cream, Recombinant

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Claudin-2 upregulation enhances intestinal permeability, immune activation, dysbiosis, and mortality in sepsis.

doi: 10.1073/pnas.2217877121

Figure Lengend Snippet: Fig. 4. Claudin-2 expression augments mucosal immune activation. (A) Representative immunofluorescent staining of myeloperoxidase-positive neutrophils (green) and F4/80-positive macrophages (red). The graphs show numbers of neutrophils and macrophages within mucosa of sham WT (green) and claudin-2 KO (light green) and CLP WT (red) and claudin-2 KO (pink) mice. (B) Mucosal CD3+CD4- (green) and CD3+CD4+ (yellow) T cell infiltration is similar across all conditions. (C) Flow cytometric plot and graph of CD8αβ IEL numbers 24 h after CLP. (D) Flow cytometric plot and graph of IL-17 producing TCRγδ IELs after CLP. (E) Cytokine mRNA expression shows that claudin-2 KO mice have reduced IL-1β and IL-6 transcription, relative to WT, at 24 h after CLP. (F) Within Peyer's patches, the fractions of CD4+CD69+, CD4+CD25+, and CD8+CD25+ lymphocytes among all CD3+ cells were significantly greater in WT, relative to claudin-2 KO, mice. n = 5 to 12 for each condition. *P < 0.05; **P < 0.01; ***P < 0.001. NaK ATPase (blue) is shown for reference (A and B). Scale bars, 100 μm, 20 μm (Insets).

Article Snippet: Primary antibodies used for immunohisto chemistry Antigen Host Source Clone/Catalog RRID Concentration Claudin- 2 Rabbit Turner lab Rb188- 1/2 AB_2916077 1 μg/mL (IF) Claudin- 3 Rabbit Thermo fisher scientific 34- 1700 AB_86804 1 μ g/mL (IF) Claudin- 4 Rabbit Abcam ab210796 AB_2732879 0.1 μg/mL (IF) Claudin- 5 Rabbit BiCell 205 1 μ g/mL (IF) Claudin- 7 Rabbit Abcam ab207300 AB_2783812 0.5 μ g/mL (IF) Claudin- 15 Rabbit BiCell 215 1 μ g/mL (IF) pMLC Rabbit Turner lab 6889+6890 AB_2916078 1 μg/mL (IF) Occludin Rat Turner lab Clone 6B8A3 AB_2819194 1 μ g/mL (IF) Occludin Rat Turner lab Clone 5E5A6 AB_2819196 1 μg/mL (IF) E- cadherin Mouse Abnova MAB1388 AB_1671631 1 μ g/mL (IF) NaKATPase Mouse SantaCruz sc- 48345 AB_626712 0.5 μg/mL (IF) ZO- 1 Rat Turner lab Clone 6B6E4 AB_2783858 0.5 μ g/mL (IF) ZO- 1 Rat Turner lab Clone R40.76 AB_2783859 0.5 μ g/mL (IF) CD3 Rabbit Abcam Ab16669 AB_443425 5 μg/mL (IF) CD4 Rat eBioscience 13- 9766- 82 AB_2572833 5 μg/mL (IF) CD8 Rat eBioscience 13- 0808- 82 AB_2572771 5 μ g/mL (IF) CD25 Mouse Novus biologicals NB600- 564 AB_10002565 5 μg/mL (IF) CD68 Mouse SantaCruz sc- 20060 AB_2891106 1 μg/mL (IF) F4/80 Rabbit Cell signaling 70076T AB_2799771 1 μ g/mL (IF) MPO Rabbit Abcam Ab9535 AB_307322 1 μ g/mL (IF) JAMA Rabbit Thermo fisher scientific 36- 1700 AB_148483 0.5 μg/mL (IF) Ki67 Rabbit Novus biologicals NB600- 1252 AB_2142376 5 μ g/mL (IF) CD3 Rat BioRad MCA1477A488 AB_321245 5 μg/mL (IF) CD8 Rat Biolegend 372902 AB_2650657 10 μ g/mL (IF)

Techniques: Expressing, Activation Assay, Staining